The function of this design is to continuously monitor the level of neurotransmitter during the study of free- moving animal learning activity. This devise is designed to gain in vivo brain electrochemical signals by composing Bluetooth wireless transmission system. Therefore, the function of this design is to continuously monitor the level of neurotransmitter during the study of free-moving animal learning activity and to give current stimulation in a conscious animal brain to activate the function of a specific brain region. This design allowed in vivo central nervous signal recording in free-moving animal without mechanical disturbance and cable disconnection during recording, which could improve the accuracy of the electrochemical recording.